Description
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with P53antigen. Standards or samples are then added to the appropriate microtiterplate wells with a biotin-conjugated P53 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain P53 antibody,biotin-conjugated P53 and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of P53 antibody in the samples is then determined bycomparing the O.D. of the samples to the standard curve